Chromogranin B Recombinant Rabbit Monoclonal Antibody [JE31-72] (HA721398)
Catalog# HA721398
Rabbit Monoclonal to Chromogranin B
Tested for
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WB
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (HA721398) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: SW620 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 120 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721398) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: SW620 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 120 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721398) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.