Chil3 / Ym-1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-49] (HA751945)
Catalog# HA751945
Rabbit Monoclonal to Chil3 / Ym-1
Tested for
-
WB
-
IHC-P
-
IF-Tissue
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Chil3 / Ym-1 on different lysates with Rabbit anti-Chil3 / Ym-1 antibody (HA751945) at 1/100,000 dilution.
Lane 1: Mouse lung tissue lysate (30 µg/Lane)
Lane 2: Mouse brain tissue lysate (30 µg/Lane)
Lane 3: Mouse bone marrow tissue lysate (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (30 µg/Lane)
Exposure time: 30 seconds; ECL: K1801
Low expression: Mouse brain (PMID: 25551794).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751945, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 44 kDa
Observed band size: 44 kDa
Western blot analysis of Chil3 / Ym-1 on different lysates with Rabbit anti-Chil3 / Ym-1 antibody (HA751945) at 1/100,000 dilution.
Lane 1: Mouse lung tissue lysate (30 µg/Lane)
Lane 2: Mouse brain tissue lysate (30 µg/Lane)
Lane 3: Mouse bone marrow tissue lysate (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (30 µg/Lane)
Exposure time: 30 seconds; ECL: K1801
Low expression: Mouse brain (PMID: 25551794).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751945, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 44 kDa
Observed band size: 44 kDa