CD80 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH18-13] (HA751659)
Catalog# HA751659
Rabbit Monoclonal to CD80
Tested for
-
WB
-
IF-Cell
-
FC
-
IP
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD80 on different lysates with Rabbit anti-CD80 antibody (HA751659) at 1/5,000 dilution.
Lane 1: C2C12 cell lysate
Lane 2: NIH/3T3 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 60 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751659) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD80 on different lysates with Rabbit anti-CD80 antibody (HA751659) at 1/5,000 dilution.
Lane 1: C2C12 cell lysate
Lane 2: NIH/3T3 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 60 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751659) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.