CD80 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-79] (HA751321)
Catalog# HA751321
Rabbit Monoclonal to CD80
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD80 on different lysates with Rabbit anti-CD80 antibody (HA751321) at 1/2,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (negative) (20 µg/Lane)
Lane 3: HDLM-2 cell lysate (20 µg/Lane)
Predicted band size: 33 kDa
Observed band size: 55-60 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751321) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD80 on different lysates with Rabbit anti-CD80 antibody (HA751321) at 1/2,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (negative) (20 µg/Lane)
Lane 3: HDLM-2 cell lysate (20 µg/Lane)
Predicted band size: 33 kDa
Observed band size: 55-60 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751321) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.