CD8 alpha Recombinant Rabbit Monoclonal Antibody [PSH19-81] (HA724105)
Catalog# HA724105
Rabbit Monoclonal to CD8 alpha
Tested for
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WB
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IHC-P
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IF-Tissue
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FC
Reacts with
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Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD8 alpha on different lysates with Rabbit anti-CD8 alpha antibody (HA724105) at 1/5,000 dilution.
Lane 1: MOLT-4 cell lysate
Lane 2: PC-3 cell lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724105) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD8 alpha on different lysates with Rabbit anti-CD8 alpha antibody (HA724105) at 1/5,000 dilution.
Lane 1: MOLT-4 cell lysate
Lane 2: PC-3 cell lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724105) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.