CD48 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-53] (HA751901)
Catalog# HA751901
Rabbit Monoclonal to CD48
Tested for
-
WB
-
FC
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA751901) at 1/5,000 dilution.
Lane 1: CTLL-2 (Mouse T lymphocytes) cell lysate
Lane 2: 3T3-L1 (Mouse embryonic fibroblasts) cell lysate
Lane 3: BaF3 (Mouse pro-B cells) cell lysate
Lane 4: A20 (Mouse B-cell lymphoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 16 seconds; ECL: K1801
CD48 exists as highly glycosylated protein on the cell surface. The molecule weight observed is consistent with what has been described in the literature (PMID: 41482455).
Low expression of CD48 protein in 3T3-L1 cell is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751901, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 27 kDa
Observed band size: 45-60 kDa
Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA751901) at 1/5,000 dilution.
Lane 1: CTLL-2 (Mouse T lymphocytes) cell lysate
Lane 2: 3T3-L1 (Mouse embryonic fibroblasts) cell lysate
Lane 3: BaF3 (Mouse pro-B cells) cell lysate
Lane 4: A20 (Mouse B-cell lymphoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 16 seconds; ECL: K1801
CD48 exists as highly glycosylated protein on the cell surface. The molecule weight observed is consistent with what has been described in the literature (PMID: 41482455).
Low expression of CD48 protein in 3T3-L1 cell is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751901, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 27 kDa
Observed band size: 45-60 kDa