CD3 Recombinant Rabbit Monoclonal Antibody (IRS074)

UNIPROT #

P22646

PROTEIN NAME

CD3

SYNONYMS

4930549J05Rik antibody;A430104F18Rik antibody;AW552088 antibody;Cd247 antibody;CD247 antigen antibody;CD247 antigen, zeta subunit antibody;CD247 molecule antibody;CD3 antibody;CD3 antigen, delta subunit antibody;CD3 delta antibody;CD3 epsilon antibody;CD3 eta antibody;CD3 gamma antibody;CD3 molecule delta polypeptide antibody;CD3 molecule, epsilon polypeptide antibody;CD3 molecule, gamma polypeptide antibody;CD3 zeta antibody;CD3-DELTA antibody;CD3d antibody;CD3D antigen delta polypeptide antibody;CD3d antigen, delta polypeptide (TiT3 complex) antibody;CD3d molecule delta antibody;CD3d molecule delta CD3 TCR complex antibody;CD3d molecule, delta (CD3-TCR complex) antibody;CD3D_HUMAN antibody;CD3E antibody;CD3e antigen antibody;CD3E antigen epsilon polypeptide antibody;CD3e antigen, epsilon polypeptide (TiT3 complex) antibody;CD3e molecule epsilon antibody;CD3e molecule epsilon CD3 TCR complex antibody;CD3e molecule, epsilon (CD3-TCR complex) antibody;CD3epsilon antibody;CD3G antibody;CD3g antigen antibody;CD3G antigen gamma polypeptide antibody;CD3g antigen, gamma polypeptide (TiT3 complex) antibody;CD3g molecule gamma antibody;CD3g molecule gamma CD3 TCR complex antibody;CD3g molecule, gamma (CD3-TCR complex) antibody;CD3H antibody;CD3Q antibody;CD3Z antibody;CD3zeta antibody;Ctg3 antibody;FLJ17620 antibody;FLJ17664 antibody;FLJ18683 antibody;FLJ79544 antibody;FLJ94613 antibody;IMD19 antibody;Leu-4 antibody;MGC138597 antibody;OKT3, delta chain antibody;OTTHUMP00000032544 antibody;T cell receptor antibody;T cell receptor T3 delta chain antibody;T cell receptor T3 gamma chain antibody;T cell receptor T3 zeta chain antibody;T cell receptor zeta chain antibody;T cell surface antigen T3/Leu 4 epsilon chain antibody;T cell surface glycoprotein CD3 antibody;T cell surface glycoprotein CD3 delta chain antibody;T cell surface glycoprotein CD3 epsilon chain antibody;T cell surface glycoprotein CD3 gamma chain antibody;T cell surface glycoprotein CD3 zeta chain antibody;T-cell antigen receptor complex, delta subunit of T3 antibody;T-cell antigen receptor complex, epsilon subunit of T3 antibody;T-cell antigen receptor complex, gamma subunit of T3 antibody;T-cell antigen receptor complex, zeta subunit of CD3 antibody;T-cell receptor T3 delta chain antibody;T-cell receptor T3 gamma chain antibody;T-cell surface antigen T3/Leu-4 epsilon chain antibody;T-cell surface glycoprotein CD3 delta chain antibody;T-cell surface glycoprotein CD3 epsilon chain antibody;T-cell surface glycoprotein CD3 gamma chain antibody;T3 antibody;T3d antibody;T3e antibody;T3g antibody;T3z antibody;TCRE antibody;TCRk antibody;Tcrz antibody;TCRzeta antibody

TISSUE SPECIFICITY

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level). Expressed on neutrophils (at protein level). Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Ser and Tyr residues after cellular activation by src kinases. Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690. Constitutively phosphorylated on Ser-734 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.; Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines. Except for a weak expression in Purkinje cells (with some of the Abs) and activated NK-cells, CD3 is found only in T-cells. CD3 appear in the cytoplasm of prothymocytes, and on the surface of about 95% of thymocytes, while cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. In therapy resistant celiac disease, a shift from membranous to cytoplasmic CD3 expression is seen (together with loss of CD8). In malignant lymphomas, CD3 is a pan-T-cell lineage-restricted antigen, detected in 80-97% of the T-cell lymphomas. Mature T-cell lymphomas including cases of mycosis fungoides, peripheral T-cell lymphoma and anaplastic large cell lymphoma may aberrantly lose CD3. NK-cell lymphomas can show a cytoplasmic reaction. Reed-Sternberg cells may show a globular paranuclear reaction. CD3 is an important marker in the classification of malignant lymphomas and lymphoid leukaemias. Also the marker is useful for the identification of T-cells in, e.g., celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein.