CD25 / IL-2 Receptor alpha Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH21-79] (HA751810)
Catalog# HA751810
Rabbit Monoclonal to CD25 / IL-2 Receptor alpha
Tested for
-
WB
-
IHC-P
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD25 / IL-2 Receptor alpha on different lysates with Rabbit anti-CD25 / IL-2 Receptor alpha antibody (HA751810) at 1/20,000 dilution.
Lane 1: CTLL-2 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (negative) (20 µg/Lane)
Lane 3: C2C12 cell lysate (negative) (20 µg/Lane)
Predicted band size: 31 kDa
Observed band size: 60 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751810) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD25 / IL-2 Receptor alpha on different lysates with Rabbit anti-CD25 / IL-2 Receptor alpha antibody (HA751810) at 1/20,000 dilution.
Lane 1: CTLL-2 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (negative) (20 µg/Lane)
Lane 3: C2C12 cell lysate (negative) (20 µg/Lane)
Predicted band size: 31 kDa
Observed band size: 60 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751810) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.