CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric) - BSA and Azide free (HA610405)
Catalog# HA610405
to CD21
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD21 on different lysates with Rabbit anti-CD21 antibody (HA610405) at 1/5,000 dilution.
Lane 1: Raji (Human Burkitt's lymphoma cells)
Lane 2: K-562 (Human chronic myelogenous leukemia cells)
Lysates/proteins at 20 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Negative expression of CD21 protein in K562 cell is consistent with the predicted expression pattern
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610405, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,00 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 113 kDa
Observed band size: 150 kDa
Western blot analysis of CD21 on different lysates with Rabbit anti-CD21 antibody (HA610405) at 1/5,000 dilution.
Lane 1: Raji (Human Burkitt's lymphoma cells)
Lane 2: K-562 (Human chronic myelogenous leukemia cells)
Lysates/proteins at 20 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Negative expression of CD21 protein in K562 cell is consistent with the predicted expression pattern
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610405, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,00 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 113 kDa
Observed band size: 150 kDa