CD204 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A8G2-R] (HA610052)
Catalog# HA610052
Mouse Monoclonal to CD204
Tested for
-
WB
-
IHC-P
-
IF-Cell
Reacts with
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Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of CD204 on different lysates with Mouse anti-CD204 antibody (HA610052) at 1/1,000 dilution.
Lane 1: THP-1 treated with 50ng/mL PMA for 72 hours whole cell lysate
Lane 2: THP-1 whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 75 kDa
Exposure time: 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610052) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD204 on different lysates with Mouse anti-CD204 antibody (HA610052) at 1/1,000 dilution.
Lane 1: THP-1 treated with 50ng/mL PMA for 72 hours whole cell lysate
Lane 2: THP-1 whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 75 kDa
Exposure time: 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610052) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.