CD19 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH13-44] (HA751463)
Catalog# HA751463
Rabbit Monoclonal to CD19
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD19 on different lysates with Rabbit anti-CD19 antibody (HA751463) at 1/50,000 dilution.
Lane 1: Raji cell lysate
Lane 2: Ramos cell lysate
Lane 3: 293T cell lysate (negative)
Lane 4: Daudi cell lysate
Lane 5: K-562 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 100 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751463) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD19 on different lysates with Rabbit anti-CD19 antibody (HA751463) at 1/50,000 dilution.
Lane 1: Raji cell lysate
Lane 2: Ramos cell lysate
Lane 3: 293T cell lysate (negative)
Lane 4: Daudi cell lysate
Lane 5: K-562 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 100 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751463) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.