CD11c Recombinant Rabbit Multiclonal Antibody - BSA and Azide free [PSH13-25] (HA751461)
Catalog# HA751461
Rabbit Multiclonal to CD11c
Tested for
-
WB
-
IHC-Fr
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA751461) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 starved for 2 hours then add 100ng/mL TPA for 48 hours cell lysate
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (negative) (20 µg/Lane)
Lane 5: Mouse colon tissue lysate (40 µg/Lane)
Lane 6: Mouse spleen tissue lysate (40 µg/Lane)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 129 kDa
Observed band size: 140 kDa
Exposure time: Lane 1-2: 59 seconds; Lane 3-5: 25 seconds; Lane 6: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751461) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA751461) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 starved for 2 hours then add 100ng/mL TPA for 48 hours cell lysate
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (negative) (20 µg/Lane)
Lane 5: Mouse colon tissue lysate (40 µg/Lane)
Lane 6: Mouse spleen tissue lysate (40 µg/Lane)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 129 kDa
Observed band size: 140 kDa
Exposure time: Lane 1-2: 59 seconds; Lane 3-5: 25 seconds; Lane 6: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751461) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.