CCL5 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-74] (HA751952)
Catalog# HA751952
Rabbit Monoclonal to CCL5
Tested for
-
WB
-
IF-Cell
-
FC(Intra)
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CCL5 on different lysates with Rabbit anti-CCL5 antibody (HA751952) at 1/5,000 dilution.
Lane 1: NK-92 (Human natural killer cells) cell lysate
Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 59 seconds; ECL: K1802
Jurkat is a negative control (PMID: 15239133).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751952, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 10 kDa
Observed band size: 10 kDa
Western blot analysis of CCL5 on different lysates with Rabbit anti-CCL5 antibody (HA751952) at 1/5,000 dilution.
Lane 1: NK-92 (Human natural killer cells) cell lysate
Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 59 seconds; ECL: K1802
Jurkat is a negative control (PMID: 15239133).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751952, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 10 kDa
Observed band size: 10 kDa