CBS Recombinant Rabbit Monoclonal Antibody [JE40-99] (ET7109-21)
Catalog# ET7109-21
Rabbit Monoclonal to CBS
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WB
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Human
Lane 1: 293T (Human embryonic kidney cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 3: MCF7 (Human breast cancer cell) cell lysate
Lane 4: LNCaP (Human prostate cancer cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7109-21, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 61 kDa
Observed band size: 61 kDa
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CBS Recombinant Rabbit Monoclonal Antibody [JE40-99] (ET7109-21)
Immunogen
Recombinant protein within human cbs aa 360-551 / 551.
Host
Rabbit
Positive Control
293T (Human embryonic kidney cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE40-99
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 61 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CBS
SYNONYMS
AI047524 antibody;AI303044 antibody;Beta thionase antibody;Beta-thionase antibody;Cbs antibody;Cbs cystathionine beta-synthase antibody;CBS_HUMAN antibody;Cystathionine beta synthase antibody;Cystathionine beta-synthase antibody;EC 4.2.1.22 antibody;HIP 4 antibody;HIP4 antibody;Methylcysteine synthase antibody;MGC18856 antibody;MGC18895 antibody;MGC37300 antibody;OTTHUMP00000109416 antibody;OTTHUMP00000109418 antibody;Serine sulfhydrase antibody
SEQUENCE SIMILARITIES
Belongs to the cysteine synthase/cystathionine beta-synthase family.
TISSUE SPECIFICITY
In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
Strongly expressed in human liver and pancreas, with weaker expression in heart and brain, the cytoplasmic protein cystathionine b-synthase (CBS) operates in the first step of homocysteine transulfuration. CBS, which belongs to the cysteine synthase/cystathionine b-synthase family of proteins, catalyzes the formation of cystathionine from the thrombogenic amino acid homocysteine using pyridoxal phosphate cofactor. Allosteric activation by adenosyl-methionine regulates CBS activity. Deficiencies in CBS are assoc-iated with homocystinuria, a recessively inherited error in sulfur amino acid metabolism that affects many organs and tissues. Symptoms of homocytinuria include arteriosclerosis, thrombosis, dislocated optic lenses, mental retard-ation and skeletal abnormalities.
CITATIONS
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Inhibition of cystathionine β-synthase promotes apoptosis and reduces cell proliferation in chronic myeloid leukemia
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WB
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Human
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Citation
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