Carbonic anhydrase 2 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [11A2-R] (HA610116)
Catalog# HA610116
Mouse Monoclonal to Carbonic anhydrase 2
Tested for
-
WB
-
IF-Cell
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IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Carbonic anhydrase 2 on different lysates with Mouse anti-Carbonic anhydrase 2 antibody (HA610116) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610116) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610116) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.