C10orf58 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [7-B7-R] (HA610088)
Catalog# HA610088
Mouse Monoclonal to C10orf58
Tested for
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WB
-
IF-Cell
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FC
Reacts with
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Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of C10orf58 on different lysates with Mouse anti-C10orf58 antibody (HA610088) at 1/2,000 dilution.
Lane 1: HUVEC cell lysate (20 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 3: Rat skin tissue lysate (40 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 24/25 kDa
Exposure time: 1 minute 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HUVEC cell lysate (20 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 3: Rat skin tissue lysate (40 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 24/25 kDa
Exposure time: 1 minute 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610088) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.