BRN3A Recombinant Antibody [PSH11-65] - Rat IgG1 (Chimeric) - BSA and Azide free (HA610252)
Catalog# HA610252
to BRN3A
Tested for
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IHC-Fr
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IHC-P
Reacts with
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Immunofluorescence analysis of frozen mouse embryo tissue with Rat anti-BRN3A antibody (HA610252) at 1/500 dilution.
The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610252, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610252, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).