Bone sialoprotein 2 Recombinant Rabbit Monoclonal Antibody [PSH09-64] (HA723129)
Catalog# HA723129
Rabbit Monoclonal to Bone sialoprotein 2
Tested for
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
Reacts with
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Bone sialoprotein 2 on different lysates with Rabbit anti-Bone sialoprotein 2 antibody (HA723129) at 1/2,000 dilution.
Lane 1: mouse articular cartilage tissue lysate
Lane 2: mouse embryo tissue lysate
Lane 3: rat embryo tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 3 minute ; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723129) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: mouse articular cartilage tissue lysate
Lane 2: mouse embryo tissue lysate
Lane 3: rat embryo tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 3 minute ; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723129) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.