Biotin Conjugated Dxd Recombinant Mouse Monoclonal Antibody [PSH0-37] (HA601134B)
Catalog# HA601134B
Mouse Monoclonal to Dxd
Tested for
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ELISA
Reacts with
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Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to Biotin
Competitive ELISA analysis of Dxd was performed by coating wells of a 96-well plate with 50 µl per well of Dxd-SMCC-CCH diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 50 µL per well of Dxd monoclonal antibody (HA601134B) at concentration of 1 µg/mL with serial diluted Dxd starting from a concentration of 10µg/mL for 1 hour at room temperature. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.