beta Tubulin Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A1-A4-R] (HA610067)
Catalog# HA610067
Mouse Monoclonal to beta Tubulin
Tested for
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WB
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IF-Cell
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FC
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IHC-P
Reacts with
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Human
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Mouse
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Rat
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Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (HA610067) at 1/10,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: A549 cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: L-929 cell lysate (10 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: C6 cell lysate (10 µg/Lane)
Lane 7: Vero cell lysate (10 µg/Lane)
Lane 8: COS-1 cell lysate (10 µg/Lane)
Lane 9: Mouse spleen tissue lysate (20 µg/Lane)
Lane 10: Mouse stomach tissue lysate (20 µg/Lane)
Lane 11: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610067) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: A549 cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: L-929 cell lysate (10 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: C6 cell lysate (10 µg/Lane)
Lane 7: Vero cell lysate (10 µg/Lane)
Lane 8: COS-1 cell lysate (10 µg/Lane)
Lane 9: Mouse spleen tissue lysate (20 µg/Lane)
Lane 10: Mouse stomach tissue lysate (20 µg/Lane)
Lane 11: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610067) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.