beta IV Tubulin Recombinant Rabbit Monoclonal Antibody [PSH17-78] (HA723947)
Catalog# HA723947
Rabbit Monoclonal to beta IV Tubulin
Tested for
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WB
-
IF-Cell
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IHC-P
-
FC
-
IP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of beta IV Tubulin on different lysates with Rabbit anti-beta IV Tubulin antibody (HA723947) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (low expression) (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Mouse spleen tissue lysate (low expression) (40 µg/Lane)
Lane 8: Rat spleen tissue lysate (low expression) (40 µg/Lane)
Lane 9: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 52 kDa
Exposure time: Lane 1-5: 25 seconds; Lane 6-9: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723947) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of beta IV Tubulin on different lysates with Rabbit anti-beta IV Tubulin antibody (HA723947) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (low expression) (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Mouse spleen tissue lysate (low expression) (40 µg/Lane)
Lane 8: Rat spleen tissue lysate (low expression) (40 µg/Lane)
Lane 9: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 52 kDa
Exposure time: Lane 1-5: 25 seconds; Lane 6-9: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723947) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.