BAF180 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH02-34] (HA750799)
Catalog# HA750799
Rabbit Monoclonal to BAF180
Tested for
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WB
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IHC-P
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
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Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of BAF180 on different lysates with Rabbit anti-BAF180 antibody (HA750799) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: SK-MEL-28 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 193 kDa
Observed band size: 193 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750799) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: SK-MEL-28 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 193 kDa
Observed band size: 193 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750799) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.