Sirtuin Antibody Sampler Kit (HAK21076)
Catalog# HAK21076
Reacts with
-
Human
Western blot analysis of SIRT3 on different lysates with Rabbit anti-SIRT3 antibody (HA722251) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: PANC-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: SiHa cell lysate
Lane 5: Mouse liver tissue lysate
Lane 6: Mouse kidney tissue lysate
Lane 7: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 28 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722251) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: PANC-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: SiHa cell lysate
Lane 5: Mouse liver tissue lysate
Lane 6: Mouse kidney tissue lysate
Lane 7: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 28 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722251) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.