Matrix Remodeling Antibody Sampler Kit (HAK21072)
Catalog# HAK21072
Reacts with
-
Human
Western blot analysis of MMP-14 on different lysates with Rabbit anti-MMP-14 antibody (ET1606-48) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: MEF cell lysate
Lane 3: L6 cell lysate
Lane 4: Mouse lung tissue lysate
Lane 5: Rat lung tissue lysate
Lysates/proteins at 20/40 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 55 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NIH/3T3 cell lysate
Lane 2: MEF cell lysate
Lane 3: L6 cell lysate
Lane 4: Mouse lung tissue lysate
Lane 5: Rat lung tissue lysate
Lysates/proteins at 20/40 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 55 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.