ATG13 Rabbit Polyclonal Antibody (HA500639)
Catalog# HA500639
Rabbit Polyclonal to ATG13
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of ATG13 on different lysates with Rabbit anti-ATG13 antibody (HA500639) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: MCF7 (Human breast cancer cell) cell lysate
Lane 3: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate
Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 5: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 6: 293T (Human embryonic kidney cell) cell lysate
Lane 7: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 8: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 42 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500639, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 57 kDa
Observed band size: 70 kDa
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: MCF7 (Human breast cancer cell) cell lysate
Lane 3: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate
Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 5: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 6: 293T (Human embryonic kidney cell) cell lysate
Lane 7: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 8: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 42 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500639, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 57 kDa
Observed band size: 70 kDa