Astrocyte Markers Antibody Sampler Kit (HAK21083)
Catalog# HAK21083
Reacts with
-
Mouse
Western blot analysis of Aquaporin 4 on different lysates with Rabbit anti-Aquaporin 4 antibody (HA722672) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat) (40 µg/Lane)
Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)
Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 35 kDa
Observed band size: 30 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (no heat) (40 µg/Lane)
Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)
Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 35 kDa
Observed band size: 30 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.