ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26] (HA721306)
Catalog# HA721306
Rabbit Monoclonal to ASC1
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WB
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IHC-P
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Human
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Rat
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: U87-MG cell lysate
Lane 4: 293T cell lysate
Lane 5: HL-60 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: RH-35 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 3 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721306) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26] (HA721306)
Immunogen
Recombinant protein within human asc1 aa 1-581 / 581.
Host
Rabbit
Positive Control
A549 cell lysate, Hela cell lysate, U87-MG cell lysate, 293T cell lysate, HL-60 cell lysate, HepG2 cell lysate, RH-35 cell lysate, HeLa cell lysate, Rat testis tissue lysate, rat small intestine tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH0-26
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 66 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ASC1
SYNONYMS
Activating signal cointegrator 1 antibody;ASC 1 antibody;ASC-1 antibody;HsT17391 antibody;Thyroid hormone receptor interactor 4 antibody;Thyroid receptor interacting protein 4 antibody;Thyroid receptor-interacting protein 4 antibody;TR-interacting protein 4 antibody;TRIP 4 antibody;TRIP-4 antibody;Trip4 antibody;TRIP4_HUMAN antibody;ZC2HC5 antibody;Zinc finger C2HC5 type antibody
SUBCELLULAR LOCATION
Nucleus, Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
FUNCTION
This gene encodes a subunit of the tetrameric nuclear activating signal cointegrator 1 (ASC-1) complex, which associates with transcriptional coactivators, nuclear receptors and basal transcription factors to facilitate nuclear receptors-mediated transcription. This protein is localized in the nucleus and contains an E1A-type zinc finger domain, which mediates interaction with transcriptional coactivators and ligand-bound nuclear receptors, such as thyroid hormone receptor and retinoid X receptor alpha, but not glucocorticoid receptor. Mutations in this gene are associated with spinal muscular atrophy with congenital bone fractures-1 (SMABF1).
CITATIONS
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Wei Shi,et al
Copper induced cytosolic escape of mitochondrial DNA and activation of cGAS-STING-NLRP3 pathway-dependent pyroptosis in C8-D1A cells
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WB
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Mouse
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Citation
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Wang Yue,et al
Artemisinin attenuated ischemic stroke induced pyroptosis by inhibiting ROS/TXNIP/NLRP3/Caspase-1 signaling pathway
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WB
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Rat
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Citation
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