Apelin Receptor Rabbit Polyclonal Antibody (HA500634)
Catalog# HA500634
Rabbit Polyclonal to Apelin Receptor
Tested for
-
WB
Reacts with
-
Mouse
-
Rat
☑ Cell treatment (CT)
Western blot analysis of Apelin Receptor on different lysates with Rabbit anti-Apelin Receptor antibody (HA500634) at 1/2,500 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse brain treated with deglycosylation tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat brain treated with deglycosylation tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 46 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500634, 1/2,500 in 5% BSA, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 43 kDa
Observed band size: 43-50 kDa
Western blot analysis of Apelin Receptor on different lysates with Rabbit anti-Apelin Receptor antibody (HA500634) at 1/2,500 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse brain treated with deglycosylation tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat brain treated with deglycosylation tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 46 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500634, 1/2,500 in 5% BSA, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 43 kDa
Observed band size: 43-50 kDa