Cell Cycle/Checkpoint Antibody Sampler Kit (HAK21137)
Catalog# HAK21137
Reacts with
-
Human
Western blot analysis of Phospho-p53 (S15) on different lysates with Rabbit anti-Phospho-p53 (S15) antibody (HA721756) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate
Lane 2: HT-29 treated with 0.5μM Doxorubicin for 24 hours cell lysate
Lane 3: HT-29 treated with 0.5μM Doxorubicin for 24 hours, then treated with λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721756) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HT-29 cell lysate
Lane 2: HT-29 treated with 0.5μM Doxorubicin for 24 hours cell lysate
Lane 3: HT-29 treated with 0.5μM Doxorubicin for 24 hours, then treated with λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721756) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.