Disulfidptosis Antibody Sampler Kit (HAK21206)
Catalog# HAK21206
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of xCT / SLC7A11 on different lysates with Rabbit anti-xCT / SLC7A11 antibody (HA724114) at 1/2,000 dilution.
Lane 1: A549 cell lysate (no heat) (20 µg/Lane)
Lane 2: Mouse cerebellum tissue lysate (no heat) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724114) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (no heat) (20 µg/Lane)
Lane 2: Mouse cerebellum tissue lysate (no heat) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724114) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.