Cell Junction Markers Antibody Sampler Kit (HAK21200)
Catalog# HAK21200
Reacts with
-
Human
Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (ET1607-75) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MDA-MB-231 cell lysate (negative)
Lane 3: HT-29 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: A431 cell lysate
Lane 6: Caco-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 80~120 kDa
Exposure time: 43 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-75) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate
Lane 2: MDA-MB-231 cell lysate (negative)
Lane 3: HT-29 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: A431 cell lysate
Lane 6: Caco-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 80~120 kDa
Exposure time: 43 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-75) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.