Toll-like Receptor Antibody Sampler Kit (HAK21199)
Catalog# HAK21199
Reacts with
-
Human
Western blot analysis of TLR7 on different lysates with Rabbit anti-TLR7 antibody (ET1704-11) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat cell lysate (hot lysis)
Lane 3: Daudi cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 121 kDa
Observed band size: 140/70 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat cell lysate (hot lysis)
Lane 3: Daudi cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 121 kDa
Observed band size: 140/70 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.