Neural Cell Markers Essentials Antibody Kit (HAK21165)
Catalog# HAK21165
Reacts with
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Human
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Mouse
-
Rat
Western blot analysis of MAP2 on different lysates with Rabbit anti-MAP2 antibody (HA723025) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse kidney tissue lysate (no heat) (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 200 kDa
Observed band size: 150-300 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723025) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse kidney tissue lysate (no heat) (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 200 kDa
Observed band size: 150-300 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723025) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.