ALP Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH14-35] (HA751517)
Catalog# HA751517
Rabbit Monoclonal to ALP
Tested for
-
WB
-
IP
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of ALP on different lysates with Rabbit anti-ALP antibody (HA751517) at 1/5,000 dilution.
Lane 1: Saos-2 cell lysate
Lane 2: RD cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: 293T cell lysate
Lane 5: C2C12 cell lysate
Lane 6: L6 cell lysate
Lane 7: Mouse heart tissue lysate
Lane 8: Mouse kidney tissue lysate (negative)
Lane 9: Rat heart tissue lysate
Lane 10: Rat kidney tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 35 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751517) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ALP on different lysates with Rabbit anti-ALP antibody (HA751517) at 1/5,000 dilution.
Lane 1: Saos-2 cell lysate
Lane 2: RD cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: 293T cell lysate
Lane 5: C2C12 cell lysate
Lane 6: L6 cell lysate
Lane 7: Mouse heart tissue lysate
Lane 8: Mouse kidney tissue lysate (negative)
Lane 9: Rat heart tissue lysate
Lane 10: Rat kidney tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 35 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751517) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.