ALDH1A3 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-87] (HA751916)
Catalog# HA751916
Rabbit Monoclonal to ALDH1A3
Tested for
-
WB
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IF-Cell
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FC
-
IP
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of ALDH1A3 on different lysates with Rabbit anti-ALDH1A3 antibody (HA751916) at 1/5,000 dilution.
Lane 1: U-87 MG (Human glioblastoma cells) cell lysate
Lane 2: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 3: MCF7 (Human breast cancer cells) cell lysate
Lane 4: 293T (Human embryonic kidney cells) cell lysate
Lane 5: Caco-2 (Human colorectal adenocarcinoma cells) cell lysate
Lane 6: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Low expression: MCF7 cells (PMID: 31197235).
Low expression of ALDH1A3 protein in 293T, Caco-2, and HeLa cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751916, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 56 kDa
Observed band size: 50-56 kDa
Western blot analysis of ALDH1A3 on different lysates with Rabbit anti-ALDH1A3 antibody (HA751916) at 1/5,000 dilution.
Lane 1: U-87 MG (Human glioblastoma cells) cell lysate
Lane 2: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 3: MCF7 (Human breast cancer cells) cell lysate
Lane 4: 293T (Human embryonic kidney cells) cell lysate
Lane 5: Caco-2 (Human colorectal adenocarcinoma cells) cell lysate
Lane 6: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Low expression: MCF7 cells (PMID: 31197235).
Low expression of ALDH1A3 protein in 293T, Caco-2, and HeLa cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751916, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 56 kDa
Observed band size: 50-56 kDa