Ak8 Rabbit Polyclonal Antibody (HA500638)
Catalog# HA500638
Rabbit Polyclonal to Ak8
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
☑ Relative expression (RE)
Western blot analysis of Ak8 on different lysates with Rabbit anti-Ak8 antibody (HA500638) at 1/200,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cells)cell lysate
Lane 2: BxPC-3 (Human primary pancreatic adenocarcinoma cells)cell lysate
Lane 3: HepG2 (Human liver cancer cells) cell lysate
Lane 4: Mouse testis tissue lysate
Lane 5: Mouse liver tissue lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 7 seconds; ECL: K1801
Negative expression of Ak8 protein in Liver is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500638, 1/200,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 55 kDa
Observed band size: 45 kDa
Western blot analysis of Ak8 on different lysates with Rabbit anti-Ak8 antibody (HA500638) at 1/200,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cells)cell lysate
Lane 2: BxPC-3 (Human primary pancreatic adenocarcinoma cells)cell lysate
Lane 3: HepG2 (Human liver cancer cells) cell lysate
Lane 4: Mouse testis tissue lysate
Lane 5: Mouse liver tissue lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 7 seconds; ECL: K1801
Negative expression of Ak8 protein in Liver is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500638, 1/200,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 55 kDa
Observed band size: 45 kDa