Agrin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH19-50] (HA751714)
Catalog# HA751714
Rabbit Monoclonal to Agrin
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Agrin on different lysates with Rabbit anti-Agrin antibody (HA751714) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: NRK cell lysate
Lane 5: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 217 kDa
Observed band size: 22/90/110/250-300 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751714) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: NRK cell lysate
Lane 5: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 217 kDa
Observed band size: 22/90/110/250-300 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751714) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.