Actin Nucleation and Polymerization Antibody Sampler Kit (HAK21079)
Catalog# HAK21079
Reacts with
-
Human
Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (HA722225) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: Huh7 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: HL-60 cell lysate
Lane 5: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722225) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: Huh7 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: HL-60 cell lysate
Lane 5: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722225) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.