Actin Rabbit Polyclonal Antibody (HA500552)
Catalog# HA500552
Rabbit Polyclonal to Actin
Tested for
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WB
Reacts with
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Arabidopsis thaliana
Western blot analysis of Actin on different lysates with Rabbit anti-Actin antibody (HA500552) at 1/2,000 dilution.
Lane 1: Oryza sativa lysate
Lane 2: Cotton lysate
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: Lane 1: 25 seconds; Lane 2: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500552) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Oryza sativa lysate
Lane 2: Cotton lysate
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: Lane 1: 25 seconds; Lane 2: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500552) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.