ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [JE56-33] (HA750469)
Catalog# HA750469
Rabbit Monoclonal to ACSL4/FACL4
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
☑ Relative expression (RE)
Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/2,000 dilution.
Lane 1: Huh7 (Human liver cancer cell) cell lysate
Lane 2: Hep G2 (Human liver cancer cell) cell lysate
Lane 3: MCF7 (Human breast cancer cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Negative expression of ACSL4 / FACL4 protein in MCF7 is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA750469, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 79 kDa
Observed band size: 75 kDa
Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (HA750469) at 1/2,000 dilution.
Lane 1: Huh7 (Human liver cancer cell) cell lysate
Lane 2: Hep G2 (Human liver cancer cell) cell lysate
Lane 3: MCF7 (Human breast cancer cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Negative expression of ACSL4 / FACL4 protein in MCF7 is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA750469, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 79 kDa
Observed band size: 75 kDa