Acetylated-Lysine Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-16] (HA751270)
Catalog# HA751270
Rabbit Monoclonal to Acetylated-Lysine
Tested for
-
WB
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IHC-P
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IF-Tissue
Reacts with
-
Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Acetylated-Lysine on different lysates with Rabbit anti-Acetylated-Lysine antibody (HA751270) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1μM TSA for 18 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Observed band size: Mutiple bands
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751270) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Acetylated-Lysine on different lysates with Rabbit anti-Acetylated-Lysine antibody (HA751270) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1μM TSA for 18 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Observed band size: Mutiple bands
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751270) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.