A-Myb Rabbit Polyclonal Antibody (HA500631)
Catalog# HA500631
Rabbit Polyclonal to A-Myb
Tested for
-
WB
Reacts with
-
Human
Western blot analysis of A-Myb on different lysates with Rabbit anti-A-Myb antibody (HA500631) at 1/2,000 dilution.
Lane 1: HCT 116 (Human colon cancer cells) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 3: Raji (Human Burkitt's lymphoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500631, 1/2,000 in 5% BSA, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 86 kDa
Observed band size: 86 kDa
Lane 1: HCT 116 (Human colon cancer cells) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 3: Raji (Human Burkitt's lymphoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500631, 1/2,000 in 5% BSA, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 86 kDa
Observed band size: 86 kDa