Phospho-MNK1 (T250 + T255) Recombinant Rabbit Monoclonal Antibody [PSH17-95] (HA723960)
Catalog# HA723960
Rabbit Monoclonal to Phospho-MNK1 (T250 + T255)
Tested for
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WB
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IHC-P
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IF-Cell
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FC
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-MNK1 (T250 + T255) on different lysates with Rabbit anti-Phospho-MNK1 (T250 + T255) antibody (HA723960) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate
Lane 3: HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lane 4: NIH/3T3 starved for 24 hours cell lysate
Lane 5: NIH/3T3 starved for 24 hours then treated with 10% FBS for 30 minutes cell lysate
Lane 6: NIH/3T3 starved for 24 hours then treated with 10% FBS for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723960) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-MNK1 (T250 + T255) on different lysates with Rabbit anti-Phospho-MNK1 (T250 + T255) antibody (HA723960) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate
Lane 3: HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lane 4: NIH/3T3 starved for 24 hours cell lysate
Lane 5: NIH/3T3 starved for 24 hours then treated with 10% FBS for 30 minutes cell lysate
Lane 6: NIH/3T3 starved for 24 hours then treated with 10% FBS for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723960) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.