Integrin beta 5 Recombinant Rabbit Monoclonal Antibody [PSH10-13] (HA723182)
Catalog# HA723182
Rabbit Monoclonal to Integrin beta 5
Tested for
-
WB
-
IHC-P
Reacts with
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Human
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Integrin beta 5 on different lysates with Rabbit anti-Integrin beta 5 antibody (HA723182) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: NCI-H1299 cell lysate
Lane 4: PANC-1 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: C6 cell lysate
Lane 7: Rat lung tissue lysate
Lane 8: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 1 minute 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723182) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: NCI-H1299 cell lysate
Lane 4: PANC-1 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: C6 cell lysate
Lane 7: Rat lung tissue lysate
Lane 8: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 1 minute 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723182) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.