LIAS Recombinant Rabbit Monoclonal Antibody [PSH04-04] (HA722085)
Catalog# HA722085
Rabbit Monoclonal to LIAS
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WB
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: LO2 cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: MCF7 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: HepG2 cell lysate (20 µg/Lane)
Lane 7: PC-3M cell lysate (20 µg/Lane)
Lane 8: C2C12 cell lysate (20 µg/Lane)
Lane 9: L6 cell lysate (20 µg/Lane)
Lane 10: Mouse liver tissue lysate (40 µg/Lane)
Lane 11: Mouse colon tissue lysate (40 µg/Lane)
Lane 12: Mouse brain tissue lysate (40 µg/Lane)
Lane 13: Rat liver tissue lysate (40 µg/Lane)
Lane 14: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 39 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722085) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
LIAS Recombinant Rabbit Monoclonal Antibody [PSH04-04] (HA722085)
Immunogen
Recombinant protein within human lias aa 1-372 / 372.
Host
Rabbit
Positive Control
K-562 cell lysate, LO2 cell lysate, Raji cell lysate, MCF7 cell lysate, Jurkat cell lysate, HepG2 cell lysate, PC-3M cell lysate, C2C12 cell lysate, L6 cell lysate, mouse liver tissue lysate, mouse colon tissue lysate, mouse brain tissue lysate, rat liver tissue lysate, rat testis tissue lysate, C2C12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH04-04
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 42 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
LIAS
SYNONYMS
HUSSY 01 antibody;LAS antibody;LIP 1 antibody;Lip syn antibody;LIP1 antibody;Lipoate synthase antibody;Lipoic acid synthetase antibody;Lipoic acid synthetase mitochondrial antibody;Lipoyl synthase, mitochondrial antibody;LS antibody;MGC23245 antibody
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
Lipoic acid synthetase is a protein that in humans is encoded by the LIAS gene. The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
CITATIONS
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Hu Jiahe,et al
Engineered-Doping Strategy for Self-Sufficient Reactive Oxygen Species Blossom to Amplify Ferroptosis/Cuproptosis Sensibilization in Hepatocellular Carcinoma Treatment
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WB
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Human
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Citation
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