6X His tag Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH07-10] (HA751132)
Catalog# HA751132
Rabbit Monoclonal to 6X His tag
Tested for
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WB
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IF-Cell
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IHC-P
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FC
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IP
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ELISA
Reacts with
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Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of 6X His tag on different lysates with Rabbit anti-6X His tag antibody (HA751132) at 1/5,000 dilution.
Lane 1: 293T transfected with His-tagged empty control cell lysate
Lane 2: 293T transfected with His-tagged ACAT2 (N-terminal) cell lysate
Lane 3: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751132) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with His-tagged empty control cell lysate
Lane 2: 293T transfected with His-tagged ACAT2 (N-terminal) cell lysate
Lane 3: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751132) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.