5-Hydroxymethylcytosine (5-hmC) Recombinant Mouse Monoclonal Antibody - BSA and Azide free [PSH06-08] (HA610209)
Catalog# HA610209
Mouse Monoclonal to 5-Hydroxymethylcytosine (5-hmC)
Tested for
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WB
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IF-Cell
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Dot Blot
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ELISA
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MeDIP
Reacts with
-
Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of 5-Hydroxymethylcytosine (5-hmC) on different conjugations with Mouse anti-5-Hydroxymethylcytosine (5-hmC) antibody (HA610209) at 1/1,000 dilution.
Lane 1: Uridine-BSA (negative)
Lane 2: Cytidine-BSA (negative)
Lane 3: 5-Methylcytosine-BSA (negative)
Lane 4: 3-Methylcytosine-BSA (negative)
Lane 5: 5-Hydroxymethylcytidine-BSA (positive)
Lysates/proteins at 2 μg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610209) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of 5-Hydroxymethylcytosine (5-hmC) on different conjugations with Mouse anti-5-Hydroxymethylcytosine (5-hmC) antibody (HA610209) at 1/1,000 dilution.
Lane 1: Uridine-BSA (negative)
Lane 2: Cytidine-BSA (negative)
Lane 3: 5-Methylcytosine-BSA (negative)
Lane 4: 3-Methylcytosine-BSA (negative)
Lane 5: 5-Hydroxymethylcytidine-BSA (positive)
Lysates/proteins at 2 μg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610209) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.