4-Hydroxynonenal Recombinant Mouse Monoclonal Antibody - BSA and Azide free [PSH23-22] (HA610401)
Catalog# HA610401
Mouse Monoclonal to 4-Hydroxynonenal
Tested for
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WB
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IF-Cell
Reacts with
-
Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of 4-Hydroxynonenal on different lysates with Mouse anti-4-Hydroxynonenal antibody (HA610401) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells）cell lysate
Lane 2: HeLa treated with 0.2mg/mL 4-HNE for 30min cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610401, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 35-150 kDa
Observed band size: 35-150 kDa
Western blot analysis of 4-Hydroxynonenal on different lysates with Mouse anti-4-Hydroxynonenal antibody (HA610401) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells）cell lysate
Lane 2: HeLa treated with 0.2mg/mL 4-HNE for 30min cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610401, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 35-150 kDa
Observed band size: 35-150 kDa